N6009
Nerve Growth Factor-2.5S from murine submaxillary gland
lyophilized powder, suitable for cell culture
Sinonimo/i:
NGF-2.5S
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About This Item
Prodotti consigliati
product name
Nerve Growth Factor-2.5S from murine submaxillary gland, NGF-2.5S, lyophilized powder, suitable for cell culture
Origine biologica
mouse
Livello qualitativo
Forma fisica
lyophilized powder
Potenza
0.1-30 ng/mL EC50 (using PC-12 cells)
PM
26.5 kDa
Confezionamento
pkg of 4X25 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
tecniche
cell culture | mammalian: suitable
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Informazioni sul gene
mouse ... Ngf(18049)
Descrizione generale
Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.
Applicazioni
Nerve Growth Factor-2.5S from murine submaxillary gland has been used:
- in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
- as a media supplement in the “process compartment” for microfluidic chamber studies
- to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression
Azioni biochim/fisiol
Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.
Altre note
2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.
Stato fisico
Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.
Risultati analitici
The biological activity is measured using a 3-day MTT assay.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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