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Merck
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Documenti fondamentali

02-101

Sigma-Aldrich

Endothelial Cell Growth Supplement, 50mg

The Endothelial Cell Growth Supplement is designed for use as a cell culture media supplement. This cell growth supplement is available in a 50 mg format & has been optimized & validated for cell culture.

Sinonimo/i:

Primary Cell Culture Supplement

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.77

Livello qualitativo

Stato

liquid

Produttore/marchio commerciale

Millipore
Upstate®

tecniche

cell culture | mammalian: suitable

input

sample type neural stem cell(s)
sample type epithelial cells

Condizioni di spedizione

dry ice

Descrizione generale

The Endothelial Cell Growth Supplement (ECGS) is designed for cell culture media supplement. This cell growth supplement ECGS is a mitogenic and is applicable for culturing mammalian, human endothelial cells, avian, smooth muscle cells, melanocytes, keratinocytes, and hybridomas either under reduced or serum-free conditions. It acts as a substitute for feeder layers in fastidious cell cultures.

Applicazioni

Endothelial Cell Growth Supplement, 50mg has been used to culture human umbilical vein endothelial cells (HUVECs).

Qualità

routinely evaluated in a 5 day growth assay of fetal bovine heart endothelial cells

Stato fisico

lyophilized from sterile water containing trace amounts of NaCl and streptomycin sulfate

Stoccaggio e stabilità

Lyophilized: 2 years at 4°C; rehydrated: 3 months at -20°C.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Eparina Grade I-A, ≥180 USP units/mg

Sigma-Aldrich

H3393

Eparina

N A Lanson et al.
Nucleic acids research, 28(15), 2882-2892 (2000-07-25)
To investigate molecular controls of cardiomyocyte proliferation, we utilized cardiomyocytes induced to proliferate indefinitely by SV40 large T antigen (T-ag). In the T-ag-immortalized AT-1, AT-2 and HL-1 cardiomyocytes, normal cellular proteins associating with T-ag and p53 were identified, isolated and
A T Kovala et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(15), 2486-2494 (2000-12-02)
The definition of signaling pathways in endothelial cells has been hampered by the difficulty of transiently transfecting these cells with high efficiency. This investigation was undertaken to develop an efficient technique for the transfection of endothelial cells for functional analyses.
H Hoshi et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 22(1), 51-56 (1986-01-01)
Endothelial cells from autopsy and biopsy specimens from a variety of adult human vascular tissue were harvested by collagenase treatment and gentle swabbing of the lumenal surface. Nutrient medium MCDB 107 containing a partially purified brain-derived growth factor (5 micrograms/ml)
S Strömblad et al.
The Journal of clinical investigation, 98(2), 426-433 (1996-07-15)
Induction of p53 activity in cells undergoing DNA synthesis represents a molecular conflict that can lead to apoptosis. During angiogenesis, proliferative endothelial cells become apoptotic in response to antagonists of integrin alphavbeta3 and this leads to the regression of angiogenic
U M Vischer et al.
Blood, 91(1), 118-127 (1998-02-07)
von Willebrand factor (vWF) is stored and released from endothelial secretory granules called Weibel-Palade (WP) bodies. Acute release can be induced by thrombin, histamine, and other mediators of thrombosis or inflammation. Their effect is thought to be mediated by an

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