TP0300
Total Protein Kit, Micro Lowry, Peterson′s Modification
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
impiego
sufficient for ~200 assays
Livello qualitativo
Descrizione generale
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
Applicazioni
Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.
Caratteristiche e vantaggi
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
Principio
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
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Contenuto correlato
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
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