TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Sinonimo/i:
Total Protein Assay Kit
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
Descrizione
wavelength 500-750nm
Livello qualitativo
impiego
sufficient for ~50 manual assays
Campo di misura della concentrazione proteica
0-1 mg/mL
Parametri
18-26 °C temperature
200 μL sample volume
Temperatura di conservazione
2-8°C
Descrizione generale
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Applicazioni
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Principio
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mLSDS
- Folin & Ciocalteu’s phenol reagent 5 mL
Richiesto ma non fornito
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I clienti hanno visto anche
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Journal of dairy science, 100(4), 2553-2563 (2017-02-22)
We isolated goat phosphopeptides via calcium and ethanol precipitation from a caseinate digest and investigated their feasibility as an iron-fortification ingredient in nutritional foods. Goat tryptic-digested phosphopeptides could bind 54.37 ± 0.50 mg of Fe/g of protein compared with goat
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