71238-M

pIEx-2 DNA - Novagen

Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

• Sinonimo/i: InsectDirect^™ System- pIEx-2 DNA
• Codice UNSPSC: 41106611
• NACRES: NA.51

Proprietà

• Livello qualitativo: 100
• Stato: liquid
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.

Attenzione

Toxicity: Standard Handling (A)

Note legali

INSECTDIRECT is a trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable