AB5612
Anti-Citrulline Antibody
serum, Chemicon®
Sinonimo/i:
CCP
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie (prevista in base all’omologia)
mammals
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunohistochemistry: suitable
Compatibilità
not suitable for immunohistochemistry (Paraffin)
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Specificità
Citrulline in glutaraldehyde fixed tissues or cells.
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
Immunogeno
Citrulline-Glutaraldehyde-BSA
Applicazioni
Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Stato fisico
Rabbit antiserum. Liquid with 0.05% sodium azide
Stoccaggio e stabilità
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation.
Willem Nesse,Johanna Westra,Jacqueline E van der Wal,Frank Abbas,Anthony P Nicholas et al.
Journal of Clinical Periodontology null
Nitric oxide modulates peristaltic muscle activity associated with fluid circulation in the sea pansy Renilla koellikeri.
Anctil, M; Poulain, I; Pelletier, C
The Journal of Experimental Biology null
Charlotte Thålin et al.
Journal of thrombosis and haemostasis : JTH, 18(10), 2732-2743 (2020-07-13)
Recent data propose a diagnostic and prognostic capacity for citrullinated histone H3 (H3Cit), a marker of neutrophil extracellular traps (NETs), in pathologic conditions such as cancer and thrombosis. However, current research is hampered by lack of standardized assays. We aimed
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