69825-M

NovaBlue Competent Cells - Novagen

Escherichia coli, rod shaped

• Sinonimo/i: Competent Cells for Transformation
• Codice UNSPSC: 41106202
• NACRES: NA.81

Proprietà

• product name: NovaBlue Competent Cells - Novagen, NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
• Origine biologica: Escherichia coli
• Livello qualitativo: 100
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Modalità di accrescimento: adherent or suspension
• Morfologia: rod shaped
• tecniche: microbiological culture: suitable
• Trasformazione cellulare: transformation efficiency: >1×10⁸ cfu/μg
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations, which result in high yields of excellent quality plasmid DNA. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacI^q repressor encoded by the F episome. Blue/white screening is not possible with NovaBlue(DE3) due to the presence of the lacZ α-peptide coding sequences in the lysogenic phage.

NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Componenti

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlNovaBlue Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2