6502

Trypsin, Bovine Pancreas

• Sinonimo/i: Trypsin, Bovine Pancreas
• Numero CAS: 9002-07-7
• Classificazione EC (Enzyme Commission): 3.4.21.4 (BRENDA, IUBMB)
• Codice UNSPSC: 12352202

Proprietà

• Forma fisica: solid
• Livello qualitativo: 100
• Attività specifica: ≥2,500 USP units/mg dry wt
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Solubilità: HCl: 1 mM; neutral buffer: soluble; water: soluble
• Attività estranea: Chymotrypsin ≤50 NF units/mg dry wt.
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.

Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.

Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.

Attenzione

Toxicity: Irritant (B)

Definizione di unità

One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.

Stato fisico

Salt-free

Ricostituzione

Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.

Altre note

Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3^rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS07,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H315 - H319 - H334 - H335
• Consigli di prudenza: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1