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48-621MAG

Millipore

MILLIPLEX® DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay

Sinonimo/i:

Cell Signaling DNA Damage Kit, DNA Damage Bead Panel, Genotoxicity Magnetic Beads

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.47

Reattività contro le specie

mouse, human, rat

Livello qualitativo

200

Produttore/marchio commerciale

Milliplex®

tecniche

multiplexing: suitable

Metodo di rivelazione

fluorometric (Luminex® xMAP®)

Temperatura di conservazione

2-8°C

Descrizione generale

The MILLIPLEX® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates:• ATR (total)• Chk1 (Ser345)• Chk2 (Thr68)• H2A.X (Ser139)• MDM2 (total)• p21 (total)• p53 (Ser15)

Specificità

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Applicazioni

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 1 (provided).The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:ATR (Total);Chk1 (Ser345);Chk2 (Thr68);H2A.X (Ser139);MDM2 (total);p21 (Total);p53 (Ser15)

Stoccaggio e stabilità

Recommended storage for kit components is 2 - 8°C.

Note legali

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Avvertenze

Danger

Indicazioni di pericolo

H302,H315,H318,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.