475911

Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

• Sinonimo/i: Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
• Numero CAS: 9003-99-0
• Classificazione EC (Enzyme Commission): 1.11.1.7 (BRENDA, IUBMB)
• Codice UNSPSC: 12352202

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: lyophilized
• Attività specifica: 150-200 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Solubilità: water: soluble (volume is lot-specific and can be found on the product label)
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• InChI: 1S/H2O3/c1-3-2/h1-2H; JSPLKZUTYZBBKA-UHFFFAOYSA-N

Descrizione

Descrizione generale

Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.

Stato fisico

Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

Nota sulla preparazione

Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.

Altre note

Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H317 - H334
• Consigli di prudenza: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classi di pericolo: Resp. Sens. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3