420291

IPTG, Animal-Free, High Purity

Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.

• Sinonimo/i: IPTG, Animal-Free, High Purity, Isopropyl-β-D-thiogalactopyranoside
• Formula empirica (notazione di Hill): C₉H₁₈O₅S
• Numero CAS: 367-93-1
• Peso molecolare: 238.30
• Numero MDL: MFCD00063273
• Codice UNSPSC: 12352201

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Impurezze: Dioxane, none detected
• Colore: white to off-white
• Solubilità: water: 100 mg/mL; methanol: soluble
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1; BPHPUYQFMNQIOC-NXRLNHOXSA-N

Descrizione

Descrizione generale

Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.

Applicazioni

• Mobile-CRISPRi as a powerful tool for modulating Vibrio gene expression.: This article explores the use of Mobile-CRISPRi for targeted gene regulation in Vibrio species, highlighting potential applications in microbial genetics and biotechnological innovations, including the manipulation of gene expression pathways facilitated by IPTG-induced systems (Geyman et al., 2024).


• A new Zymomonas mobilis platform strain for the efficient production of chemicals.: This research describes the development of a Zymomonas mobilis strain engineered for enhanced chemical production, utilizing IPTG for controlled expression of metabolic pathways, showcasing significant implications for biofuel production and industrial biotechnology (Frohwitter et al., 2024).


• Biophysical characterization and insights into the oligomeric nature of CD2-associated protein.: The study provides a detailed biophysical analysis of CD2-associated protein, employing IPTG-induced expression to elucidate its oligomeric structure and function in cellular signaling, offering insights into therapeutic target development (Qadri et al., 2024).


• Effective delivery of anti-PD-L1 siRNA with human heavy chain ferritin (HFn) in acute myeloid leukemia cell lines.: This research utilizes IPTG-inducible systems to study the delivery of therapeutic siRNAs in leukemia treatment, demonstrating the potential of IPTG in enhancing the efficacy of gene therapy approaches in oncology (Rajabinejad et al., 2024).


• Heterologous expression of nattokinase in E. coli: Biochemical characterization and functional analysis of fibrin binding residues.: This article details the heterologous expression of nattokinase in E. coli, facilitated by IPTG induction, focusing on its potential applications in thrombolytic therapy and its biochemical properties relevant to medical biochemistry (Jain et al., 2024).

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Altre note

Lama, J., et al. 1992. Gene117, 185.
Zhang, Y., and Moss, B. 1991. Natl. Acad. Sci. USA88, 1511.
Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347.
Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598.
Lee, S.G., et al. 1988. Biochemistry27, 2983.
Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable