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Documenti fondamentali

07-604-I

Sigma-Aldrich

Anti-Rac2 Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

Ras-related C3 botulinum toxin substrate 2, GX, p21-Rac2, Small G protein

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

human, mouse

tecniche

immunohistochemistry: suitable (paraffin)
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

ambient

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... RAC2(5880)

Descrizione generale

Ras-related C3 botulinum toxin substrate 2 (UniProt P15153; also known as GX, p21-Rac2, Small G protein) is encoded by the RAC2 gene (Gene ID 5880) in human. Rho family of small GTPases are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Rac1 and Rac2 share high sequence homology (90%) and are both expressed in the hematopoietic system, where they play crucial roles in mediating the interactions between hematopoietic stem cells (HSCs) and their microenvironment. Rac proteins transduce cell surface signaling events initiated by tyrosine kinase receptors, G protein-coupled receptors, and cell-to-cell contacts by acting as molecular switches, cycling between an inactive GDP-bound state and a GTP-bound active state. In addition to overlapping functions, Rac1 and Rac2 also mediate unique functions via interactions with specific partners. SAM50 and Metaxin1, for example, are among mitochondrial proteins identified to be specifically associated with Rac2. Rac activity inhibition is reported to effectively target primary human AML and CML cells and prevent disease initiation in various murine leukemia models.

Specificità

This rabbit polyclonal antibody was raised against recombinant full-length human Rac2 pro-form (a.a. 1-192) and therefore will recognize both the pro- and mature (a.a. 1-189) Rac2.

Immunogeno

GST-tagged recombinant full-length human Rac2 pro-form.

Applicazioni

Detect Rac2 using this rabbit polyclonal Anti-Rac2, Cat. No. 07-604-I, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Rac2 in human tonsil, human small intestine, and mouse spleen tissue sections.
Research Category
Signaling

Qualità

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Rac2 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.

Descrizione del bersaglio

~21 kDa observed. 21.12/21.43 kDa (human mature/pro-form) and 21.13/21.44 kDa (mouse mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 07-604

Stato fisico

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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