MABC592
Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free
clone TA99, 1 mg/mL, from mouse
Sinonimo/i:
5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
TA99, monoclonal
Reattività contro le specie
human, mouse
Concentrazione
1 mg/mL
tecniche
blocking: suitable (interfering antibodies)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2aκ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... TYRP1(7306)
Descrizione generale
TRP1/TYRP1, also known as 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA, Catalase B, Glycoprotein 75, Melanoma antigen gp75, or Tyrosinase-related protein 1 (TRP/TRP-1/TRP1), and encoded by the gene TYRP1/CAS2/TYRP/TYRRP, is involved in melanin synthesis and is the enzyme that oxidizes DHICA into indole-5,6-quinone-2-carboxylic acid in the pathway. TRP1/TYRP1 is critical for skin/eye/hair pigmentation. Mutations are associated with Albinism. TRP1/TYRP1 is regulated by the microphthalmia-associated transcription factor (MITF). MITF is a master regulator of pigmentation and a target of β-catenin in Wnt/β-catenin signaling during melanocyte differentiation. TRP1/TYRP1 is also regulated by the microRNA miR-145, and this microRNA is being examined as a possible therapy for hyperpigmentation. TRP1/TYRP1 is expressed as a membrane protein in melanosomes and endosomes in pigment cells.
Immunogeno
Whole cells corresponding to human TRP1/TYRP1.
Applicazioni
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TRP1/TYRP1 in human melanoma tissue.
Blocking of Interferring Antibodies Analysis: A representative lot from an independent laboratory suppressed the growth of subcutaneous B16 tumors (Patel, D., et al. (2008). Anticancer Res. 28(5A):2679-2686.).
Activity Assay Analysis: A representative lot from an independent laboratory improves anti-tumor efficacy by augmenting systemic CD8+T cell responses to tumor cells (Saenger, Y. M., et al. (2008). Cancer Res. 68(23):9884-9891.).
Immunoprecipiptation Analysis: A representative lot from an independent laboratory immunoprecipiated TRP1/TYRP1 from B16 cell lysate (Srinivasan, R., et al. (2002). Cancer Immun. 19(2):8.).
Blocking of Interferring Antibodies Analysis: A representative lot from an independent laboratory suppressed the growth of subcutaneous B16 tumors (Patel, D., et al. (2008). Anticancer Res. 28(5A):2679-2686.).
Activity Assay Analysis: A representative lot from an independent laboratory improves anti-tumor efficacy by augmenting systemic CD8+T cell responses to tumor cells (Saenger, Y. M., et al. (2008). Cancer Res. 68(23):9884-9891.).
Immunoprecipiptation Analysis: A representative lot from an independent laboratory immunoprecipiated TRP1/TYRP1 from B16 cell lysate (Srinivasan, R., et al. (2002). Cancer Immun. 19(2):8.).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free is validated for use in western blotting, IHC, blocking of inteferring antibodies, IP & flow cytometry for the detection of TRP1/TYRP1.
Qualità
Evaluated by Western Blottng in mouse skin tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TRP1/TYRP1 in 10 µg of mouse skin tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TRP1/TYRP1 in 10 µg of mouse skin tissue lysate.
Descrizione del bersaglio
~60 kDa observed
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Improved tumor immunity using anti-tyrosinase related protein-1 monoclonal antibody combined with DNA vaccines in murine melanoma.
Saenger, Yvonne M, et al.
Cancer Research, 68, 9884-9891 (2008)
Ling Jiang et al.
Journal of cellular physiology, 234(12), 22799-22808 (2019-05-23)
Our previous study found that Ganoderma lucidum polysaccharide (GLP), bioactive ingredients from Ganoderma lucidum, protected fibroblasts from photoaging. However, whether GLP can affect melanogenesis in melanocytes through regulating paracrine mediators that secreted by keratinocytes and fibroblasts is unclear. We aimed
Induction of autoantibodies against tyrosinase-related proteins following DNA vaccination: unexpected reactivity to a protein paralogue.
Srinivasan, Roopa, et al.
Cancer Immunity, 2, 8-8 (2002)
Enhanced suppression of melanoma tumor growth and metastasis by combined therapy with anti-VEGF receptor and anti-TYRP-1/gp75 monoclonal antibodies.
Patel, Dipa, et al.
Anticancer research, 28, 2679-2686 null
Shiyao Pei et al.
The Journal of investigative dermatology, 140(1), 152-163 (2019-07-06)
The long noncoding RNA UCA1 was first discovered in bladder cancer and is known to regulate the proliferation and migration of melanoma. However, its role in melanogenesis is unclear. In this study, we aimed to explore the role and mechanism
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
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