07-478
Anti-SNF2β/BRG1 Antibody
serum, Upstate®
Sinonimo/i:
Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, SNF2-beta, BRG-1, Mitotic growth and transcription activator, Brahma protein homolog 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A m
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
rat, mouse, human
Confezionamento
antibody small pack of 25 μL
Produttore/marchio commerciale
Upstate®
tecniche
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... SMARCA4(6597)
Descrizione generale
The SNF2 family of proteins are ATP dependant chromatin remodeling enzymes. The gene encoding this protein is also refered to as SMARCA4.
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
Specificità
This antibody recognizes the Near N-Terminus of SNF2B
Immunogeno
GST-tagged recombinant protein corresponding to the Near N-Terminus of human SNF2β.
Applicazioni
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected BRG1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Transcription Factors
Cell Cycle, DNA Replication & Repair
Transcription Factors
Use Anti-SNF2β/BRG1 Antibody (rabbit polyclonal antibody) validated in IP, WB to detect SNF2β/BRG1 also known as Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, SNF2-beta, BRG-1.
Qualità
Evaluated by Western Blotting in HELA NE cell lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected SNF2B/BRG1 in 10 µg of HELA NE cell lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected SNF2B/BRG1 in 10 µg of HELA NE cell lysate.
Descrizione del bersaglio
200 kDa
Stato fisico
Rabbit polyclonal in buffer containing with 0.05% sodium azide with 30% glycerol.
Unpurified
Stoccaggio e stabilità
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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