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Key Documents

07-448

Sigma-Aldrich

Anti-monomethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Sinonimo/i:

H3K27me1, Histone H3 (mono methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me1

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

human, vertebrates

Produttore/marchio commerciale

Upstate®

tecniche

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

monomethylation (Lys27)

Informazioni sul gene

human ... HIST1H3F(8968)

Descrizione generale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificità

Broad species cross-reactivity expected, based on sequence homology.
Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Immunogeno

Epitope: Lys27
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Applicazioni

Anti-monomethyl-Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody to detect Histone H3 monomethylated at lysine 27. This purified Ab, also known as Anti-H3K27me1 is specificity verified by dot blot (DB), published in peer reviewed journals and validated in ICC, WB, ChIP, ChIP-seq.
Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Qualità

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Descrizione del bersaglio

17 kDa

Stato fisico

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa acid extract

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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An alternative polyadenylation mechanism coopted to the Arabidopsis RPP7 gene through intronic retrotransposon domestication.
Tsuchiya, T; Eulgem, T
Proceedings of the National Academy of Sciences of the USA null
Quantitative mass spectrometry of histones H3.2 and H3.3 in Suz12-deficient mouse embryonic stem cells reveals distinct, dynamic post-translational modifications at Lys-27 and Lys-36.
Jung, HR; Pasini, D; Helin, K; Jensen, ON
Molecular and Cellular Proteomics null
Quantitative proteomics reveals that the specific methyltransferases Txr1p and Ezl2p differentially affect the mono-, di- and trimethylation states of histone H3 lysine 27 (H3K27).
Zhang, C; Molascon, AJ; Gao, S; Liu, Y; Andrews, PC
Molecular and Cellular Proteomics null
Yu Yang et al.
Nucleic acids research, 45(1), 181-197 (2016-09-28)
Epigenetic regulation is important for organismal development and response to the environment. Alteration in epigenetic status has been known mostly from the perspective of enzymatic actions of DNA methylation and/or histone modifications. In a genetic screen for cellular factors involved
Dean Sanders et al.
Plant physiology, 173(4), 2243-2252 (2017-02-17)
Epigenetic modifications play critical roles in diverse biological processes. Histone Lys-to-Met (K-to-M) mutations act as gain-of-function mutations to inhibit a wide range of histone methyltransferases and are thought to promote tumorigenesis. However, it is largely unknown whether K-to-M mutations impact

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