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Merck
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Key Documents

04-281

Sigma-Aldrich

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

clone 5F10, Upstate®, from mouse

Sinonimo/i:

ERBB, ERBB1, epidermal growth factor receptor

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

5F10, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Upstate®

tecniche

western blot: suitable

Isotipo

IgG1

N° accesso NCBI

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

N° accesso UniProt

P00533

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

phosphorylation (pThr669)

Informazioni sul gene

human ... EGFR(1956)

Specificità

Recognizes EGFR when phosphorylated at Threonine 669.

Immunogeno

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.

Applicazioni

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Qualità

Routinely evaluated by immunoblot.

Descrizione del bersaglio

180 kDa

Stato fisico

100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified
Thiophilic adsoption and size exclusion chromatography

Stoccaggio e stabilità

6 months at -20°C from date of shipment

Risultati analitici

Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A
Oncogene, 25, 7381-7390 (2006)
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al
The Journal of Biological Chemistry, 265, 12820-12827 (1990)
G J Heisermann et al.
The Journal of biological chemistry, 263(26), 13152-13158 (1988-09-15)
The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which
F Yan et al.
Oncogene, 36(50), 6919-6928 (2017-09-05)
Overexpression of DNMT1 and KIT is prevalent in lung cancer, yet the underlying molecular mechanisms are poorly understood. While the deregulated activation of DNMT1 or KIT has been implicated in lung cancer pathogenesis, whether and how DNMT1 and KIT orchestrate

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