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Organoids

Fluorescent staining of Human iPS cell-derived Colon Organoids, SCC300

Organoids are complex collections of cells grown in a 3D culturing medium that recapitulate many of the physiological and genomic features of various tissues or organs. Organoids are derived either from embryonic stem cells (ESC), induced pluripotent stems cells (iPSCs) or adult stem cells (ASCs) and differentiate through a series of controllable signaling cues into a diverse set of cell types resembling those found in their respective physiological tissues. 

Once culturing and differentiation conditions are established for a particular organoid type, they can be reproducibly created by researchers employing basic cell culturing methodologies. In the previous decade there has been an explosion of exciting advancements in this field including the generation and characterization of new organoid types, as well as applications for these organoids to advance basic biological research and in the development of novel therapeutics.  

Organoid Applications

Organoids can be valuable tools for translational studies including the development of personalized medicines where the culture of in vitro models from individual human subjects may permit the development of therapeutics tailored to individual patients.

Because of their reproducible nature, the characterization of genomic, proteomic, morphological and functional features of organoids sourced from different individuals (or from cells derived from different tissues from the same patient) can be studied in parallel. In particular, organoids used to study cancers (often called spheroids) have been found to conserve certain features found in their source tumor samples. Organoids can be used in combination with other advanced research techniques including microfluidic culturing devices like the ONIX microfluidic system to develop advanced organ-on-a-chip models, or with gene-editing tools like CRISPR to further refine the model.

3dGRO® Human Colonic Organoids

Human iPSC-derived colon organoids,  related media and expert, validated protocols are for researchers who need a controllable, accessible and reproducible model for the human intestinal epithelium. These organoids provide the structural complexity and genetic/proteomic diversity representative of native human colon tissue. Visit this technical protocol to read more about differentiation of iPSC to colon organoids or to purchase colon organoids.  

3dGRO®  Human Lung Organoid Culture System

Human iPSC-derived lung organoids, cultured with the 3dGRO™ Human Lung Organoid Culture System

Our Human iPSC-derived lung organoid differentiation system provides researchers with a controllable, accessible and reproducible system for culturing an organoid model of human lung epithelium. This system is a validated method for generating a 3D, branching airway model that reflects the structural organization and cellular heterogeneity─including the expression of pulmonary markers─of the human lung epithelium. For more information and to purchase lung organoid products, please visit here.

3dGRO® Patient-Derived Organoid Gastrointestinal Biobank

This collection of highly characterized, cryopreserved organoids is ethically sourced from patient samples. The biobank encompasses samples from multiple donors, extracted from various regions of the gastrointestinal tract, and representing both healthy and diseased tissue. These samples provide ready-to-use PDOs with optimized culture media for researchers investigating basic biology and drug development relevant to the human stomach, small intestine or colon. To learn more about this validated collection, read our technical article here.

3dGRO® PATIENT-DERIVED ORGANOID LUNG BIOBANK

Our 3dGRO® human lung organoids are an ideal model for researchers studying lung cancer and the responses patients have to chemotherapies. Ethically sourced from multiple donors, this authenticated organoid portfolio includes adenocarcinoma and squamous cell carcinoma organoid lines from patient-derived xenografts, metastatic patient tissues, or primary tumors. This collection consists of healthy or diseased organoid lines of multiple genders, age, and mutational profiles. To learn more about this validated collection, read our technical article here.

3dGRO® Patient-Derived Organoid Pancreatic Ductal Adenocarcinoma Biobank 

Our new collection of 3dGRO® pancreatic PDOs includes human pancreatic cancer organoids for life science and disease modeling research. This tissue-derived organoid biobank contains highly characterized pancreatic ductal adenocarcinoma organoid lines (PDAC organoids) from wild-type and PDAC-diseased patients with KRAS driver mutations. Visit our technical article for more information about the PDAC organoid biobank.

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