Patient-derived organoids (PDOs) are powerful three-dimensional (3D) cell culture models for studying human physiology and disease. Compared to traditional patient-derived xenograft (PDX) and two-dimensional (2D) cell models, PDOs are more physiologically relevant and can closely recapitulate tissue heterogeneity and patient responses to drugs and chemotherapeutics.
We now offer a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients, which have been developed by researchers at the University of Michigan Center for Gastrointestinal Research (UMCGR). These stomach, small intestine (duodenum, ileum), colon and rectum samples encompass diseases such as cancer (adenoma) and ulcerative colitis (UC), and augment our current offering of organoids, specialized tools, cultureware, harmonized media and reagents for 3D organoid culture.
PDOs derived from adult patient biopsies or resected tissues containing native LGR5+ stem cell populations can be cultured within an ECM-rich substrate, using specialized organoid media such as L-WRN conditioned media (SCM105). Biobank samples span a wide range of patient demographics (age and sex), include a diversity of tissues and diseases, and are:
Figure 1. Patient-derived colon organoids from both normal (SCC311, SCC321, SCC313, SCC317, SCC325) and diseased patients (rectal adenocarcinoma, SCC310) cultured in L-WRN conditioned media (SCM105) for five passages.
Ordering Information for PDO Biobank