推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1D19, recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 13.83 kDa
observed mol wt ~14 kDa
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
ELISA: suitable
affinity binding assay: suitable
western blot: suitable
同型
IgG
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1D19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Phosphohistidine phosphatase 1 (PHTP1).
免疫原
His-tagged full-length recombinant human Phosphohistidine phosphatase 1 (PHPT1).
應用
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHPT1 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHPT1 in A549 cell lysate.
ELISA Analysis: 300 μg/mL from a representative lot detected PHPT1 protein in an indirect ELISA.
Affinity Binding Assay:: A representative lot of this antibody bound PHPT1 protein with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHPT1 in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHPT1 in A549 cell lysate.
ELISA Analysis: 300 μg/mL from a representative lot detected PHPT1 protein in an indirect ELISA.
Affinity Binding Assay:: A representative lot of this antibody bound PHPT1 protein with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PHPT1, clone 1D19 ZooMAb®, Cat. No. ZRB1056, is recombinant Rabbit monoclonal antibody that detects phosphohistidine phosphatase 1 (PHPT1) and is tested for use in Affinity Binding Assay, ELISA, and Western Blotting.
標靶描述
14 kDa phosphohistidine phosphatase (UniProt: Q9NRX4; also known as EC:3.9.1.3, Phosphohistidine phosphatase 1, PHPT1, Protein histidine phosphatase, PHP, Protein janus-A homolog) is encoded by the PHPT1 (also known as PHP14, CGI-202, HSPC141) gene (Gene ID: 29085) in human. Protein histidine phosphorylation is reported to exist widely in vertebrates and PHPT is the only phosphohistidine phosphatase described in mammals. It regulates phosphohistidine levels of several proteins including those involved in signaling, lipid metabolism, and potassium ion transport. It is expressed abundantly in heart and skeletal muscle and is also found in several other tissues. Human PHPT1 is composed of six β-strands that are flanked by two -helices. The substrate-binding site is shown to be positioned between two loops, one between 1 and β4, and the other between β5 and 2. This site contains multiple residues that are shown to be important for substrate binding and include Glu51, Tyr52, His53, Tyr93, and Met95. The active site His53, located at the beginning of helix 1, is identified as the catalytic residue and the NH groups of His53, Ala54, and Ala86 and the OH group of Ser94 act as anchors of substrate and stabilize phosphate binding to the active site. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Martin, DR., et al. (2016). Sci. Rep. 6; Article 23658; Gong, W. B. et al. Biochem. J. 418(2); 337-344; Busam, R. D. et al. (2006). J. Biol. Chem. 281(45); 33830-33834).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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