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用途
sufficient for 12 labeling reactions (10 ng to 3 μg per assay)
sufficient for 24 blots (blots of 100 cm2)
品質等級
製造商／商標名
Roche
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
環保替代類別
儲存溫度
−20°C
一般說明
DIG-High Prime DNA标记和检测起始试剂盒II是一种便捷试剂盒，用于通过地高辛(DIG)-11-脱氧尿苷三磷酸(dUTP)随机引物标记DNA模板，对DIG标记杂交进行碱不稳定性和化学发光检测。该试剂盒的组装就是以便捷性为宗旨，提供即用型的CSPD并带有可用于简易应用的滴液装置、成品的封闭溶液以及DIG Easy Hyb颗粒。DIG-High Prime混合物包括稳定的Klenow酶、核苷酸、引物和反应缓冲液——所有成分均溶于一种方便的试剂中。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品为一种安全的化学品。 DIG系统是灵敏且具有成本效益的方案，可替代核酸放射性标记和检测。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。
應用
DIG-High Prime DNA标记和检测起始试剂盒II已用于多种杂交技术：
- Southern印迹
- Nrthern印迹
- 斑点印迹
- 菌落和噬菌斑杂交
- 所有类型的过滤杂交
- 总基因组DNA的单拷贝基因检测，甚至可检测高度复杂的生物体样品，如人类、大麦和小麦
包裝
1个试剂盒包含7种组分。
原則
DIG-High Prime DNA标记及检测启动装II使用地高辛（DIG），一种类固醇半抗原，对DNA探针进行标记用于杂交以及后续通过酶免疫检测的化学发光检测。“随机结合的”DNA标记方法首先由Feinberg和Vogelstein开发，基于将所有可能序列的寡核苷酸杂交至待标记的变性DNA。起始加入的DNA仅作为标记DNA的合成模板并在反应过程中不会降解，使得利用该方法能够对最低量的DNA（10 ng）进行标记。互补的DNA链通过Klenow聚合酶利用随机的寡核苷酸的3′-OH端作为引物进行合成。存在与反应中标记了地高辛的修饰后脱氧核糖核苷三磷酸被插入至新合成的互补DNA链中。
其他說明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- DIG-High Prime 5x concentrated
- DIG-labeled Control DNA, pBR328 (linearized with Bam HI) 5 μg/ml
- DNA Dilution Buffer
- Anti-Digoxigenin-AP Conjugate antibody
- CSPD ready-to-use
- Blocking Solution 10x concentrated
- DIG Easy Hyb Granules
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Skin Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
does not flash
閃點（°C）
does not flash
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商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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