ZMS1096

Anti-Sulfotyrosine Antibody, clone Sulfo-1C-A2 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: Sulfo-1C-A2, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 55 kDa; observed mol wt ~55 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG2aκ
• 表位序列: Unknown
• Protein ID登錄號: Unknown
• UniProt登錄號: Unknown
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone Sulfo-1C-A2 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detects sulfated tyrosine residues on various proteins. It does not recognize phosphotyrosine or unmodified tyrosines.

免疫原

Developed using a phage display library as described in Kehoe, JW., et al. (2006). Mol. Cell. Proteomics. 5(12); 2350-2563. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC.

應用

Quality Control Testing

Evaluated by Western Blotting with Bovine Fibrinogen.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Sulfotyrosine in Bovine Fibrinogen, but not in Fibrinogen treated with Sodium Acetate and Abalone Sulfatase.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Sulfotyrosine in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lo detected Sulfotyrosine in Human umbilical vein endothelial cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Protein tyrosine O-sulfation is a post-translational modification mediated by two Golgi tyrosylprotein sulfotransferases (TPST1 and TPST2) that catalyze the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins. These enzymes catalyze the transfer of sulfate from 3 -phosphoadenosine 5 -phosphosulfate to tyrosine residues in proteins. TPST 1 and 2 are type II transmembrane proteins that reside in the trans-Golgi network and have luminally oriented catalytic domain. Hence, tyrosine sulfation occurs only on soluble and transmembrane proteins that transit the Golgi en route to either secretion or incorporation into the plasma membrane. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosine residues in the total protein content of a cell can be sulfated. Tyrosine sulfation is shown to be essential for several biological processes, such as optimal proteolytic processing of progastrin, proteolytic activation of coagulation factors V and VIII by thrombin, proteolysis of the complement C4a-chain by C1s. Sulfotyrosine is also reported to be involved in the entry of several parasites and viruses into cells. Tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Huang, C., et al. (2007). Science. 317(5846); 1930-1934; Kehoe, JW., et al. (2006). Mol. Cell. Proteomics. 5(12); 2350-2563; Hoffhines, AJ., et al. (2006). J. Biol. Chem. 281(49); 37877-37887).

外觀

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable