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Y1771

Sigma-Aldrich

酵母营养缺陷型培养基补充剂

without uracil, leucine, and tryptophan

别名:

酵母营养缺陷型培养基补充剂, 酵母选择营养缺陷型混合物（Synthetic yeast drop-out mix）

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About This Item

MDL號碼:
MFCD01095679
分類程式碼代碼:
41106212
NACRES:
NA.85

品質等級

200

形狀

powder

技術

transformation: suitable

應用

microbiology

儲存溫度

room temp

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應用

酵母氨基酸缺陷型合成培养基添加剂不含尿嘧啶、亮氨酸和色氨酸，可用于筛查：

  • 携带pCRISPRyl_URA3sgRNA 质粒的 PO1f 菌株的转化株
  • 解脂耶氏酵母 PO1f 菌株的阳性转化株
  • CRISPR和 RNA辅助体内定向进化（CRAIDE）突变体
由于在缺乏特定培养基成分（比如氨基酸、嘌呤或嘧啶）的情况下无法生长，营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化，可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基，可以提高产量和生长速度，并增加筛选文库或进行基因敲除时转化的成功率。

其他說明

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。

準備報告

每升培养基溶解10.4克添加剂。

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D -(+)-葡萄糖, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

细菌琼脂, for molecular biology

Y1501

酵母营养缺陷型培养基补充剂, without uracil

Y2001

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan and uracil

Y0626

不含氨基酸的酵母氮源基, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1896

酵母营养缺陷型培养基补充剂, without lysine

Y2021

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan, and adenine

Y2146

酵母营养缺陷型培养基补充剂, without histidine, leucine, and tryptophan

象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H318

危險分類

Eye Dam. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps

商品

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

实验方案

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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