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Y0750

Sigma-Aldrich

酵母营养缺陷型培养基补充剂

without leucine and tryptophan

别名:

Synthetic Yeast Nutritional Supplements

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About This Item

MDL號碼:
MFCD01095679
分類程式碼代碼:
12000000
NACRES:
ND.02

應用

由于在缺乏特定培养基成分（比如氨基酸、嘌呤或嘧啶）的情况下无法生长，营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化，可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基，可以提高产量和生长速度，并增加筛选文库或进行基因敲除时转化的成功率。

成分

除非另有说明，否则补充剂包含以下所有成分：
氨基酸： 除亮氨酸和色氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76 mg/L。
其他营养素： 腺嘌呤 (18 mg/L)、肌醇 (76 mg/L)、对氨基苯甲酸 (8 mg/L)、尿嘧啶 (76 mg/L)。

其他說明

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。

數量

1 L 培养基将补充1.54 g。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
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Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

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