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Merck

UPS2

Sigma-Aldrich

蛋白质组学动态范围标准品套装

Protein Mass Spectrometry Calibration Standard

别名:

Dynamic Range Standards, Proteomics Standard Set

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.24

生物源

human

品質等級

形狀

ready-to-use solution

品質

Protein Mass Spectrometry Calibration Standard

濃度

10.6 μg/ampule protein

技術

mass spectrometry (MS): suitable

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

蛋白质组学动态范围标准品套装由48种单独的人源或人序列重组蛋白的混合物产生,每种重组蛋白都被选择用于限制异源翻译后修饰(PTM)。蛋白质标准品由6种蛋白质的6种混合物配制而成,其动态范围为5个数量级,范围为50皮摩尔至500摩尔。在配制之前,通过氨基酸分析(AAA)对每种蛋白质进行定量。

應用

蛋白组学动态范围标准套件用于基于强度的绝对定量(iBAQ),适用 大肠杆菌 蛋白、 胚胎干细胞(ESC)和神经元前体细胞(NPC)蛋白组。 也作为标准品,无标记定量刺突 HeLa 细胞。
蛋白质组学动态范围标准品套装可用于在分析复杂蛋白质样品之前标准化和/或评估质谱(例如,LC-MS / MS,MALDI-TOF-MS等)和电泳分析条件。UPS2 可用于在已知复杂标准样品的运行之间交叉包含宝贵的实验数据集。从而确认分析方法的耐用性和所用仪器的稳定性。此外,生成或比较来自定义不明确的样品的质谱数据的实验室可以使用UPS2作为外部参考,以协助评估结果和实验方法。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS(液相色谱 - 质谱)/ MS分析中基于强度的蛋白质绝对定量(iBAQ)的标准。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg化学品安全说明书

訊號詞

Danger

危險分類

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Lee Dicker et al.
Molecular & cellular proteomics : MCP, 9(12), 2704-2718 (2010-09-09)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics provides a wealth of information about proteins present in biological samples. In bottom-up LC-MS/MS-based proteomics, proteins are enzymatically digested into peptides prior to query by LC-MS/MS. Thus, the information directly available from the LC-MS/MS
Ozren Bogdanović et al.
Nature genetics, 48(4), 417-426 (2016-03-02)
The vertebrate body plan and organs are shaped during a conserved embryonic phase called the phylotypic stage. However, the mechanisms that guide the epigenome through this transition and their evolutionary conservation remain elusive. Here we report widespread DNA demethylation of
PANDA: A comprehensive and flexible tool for quantitative proteomics data analysis
Chang C, et al.
Bioinformatics, 35, 898-900 (2018)
Christina Ludwig et al.
Molecular & cellular proteomics : MCP, 11(3), M111-M111 (2011-11-22)
For many research questions in modern molecular and systems biology, information about absolute protein quantities is imperative. This information includes, for example, kinetic modeling of processes, protein turnover determinations, stoichiometric investigations of protein complexes, or quantitative comparisons of different proteins
Minia Antelo-Varela et al.
Analytical chemistry, 91(18), 11972-11980 (2019-08-20)
The field of systems biology has been rapidly developing in the past decade. However, the data produced by "omics" approaches is lagging behind the requirements of this field, especially when it comes to absolute abundances of membrane proteins. In the

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Standardize Your Research. We offer both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry.

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