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形狀
liquid
技術
liquid chromatography (LC): suitable
儲存溫度
−20°C
一般說明
凝胶过滤色谱是一种广泛使用的检测蛋白质大小和分子量的方法。蛋白质大小的差别使其进入树脂孔穴内的程度也不同，从而实现分离。较大的蛋白质不易进入树脂孔，因此比较小蛋白质更快地通过柱子。最终，这些蛋白质分子按分子量递减的顺序从柱中洗脱。
應用
凝胶过滤标记物试剂盒（蛋白分子量29,000-700,000 Da）用于：
- 蛋白纯化和分析（凝胶过滤实验）
- 突触神经小体提取物的体积排阻色谱/凝胶过滤色谱
- 甘油梯度离心
- 研究糖皮质诱导淋巴细胞溶解，作为免疫系统中细胞凋亡的模式体系
- 分离玉竹凝集素，其对Vero细胞具有显著的抗HSV-II活性，对A375人黑素瘤细胞具有细胞毒性，并可以半胱天冬酶依赖性方式诱导细胞凋亡
生化／生理作用
手册所述凝胶过滤色谱法测定分子量是现有发表方法的改良版。该试剂盒中的蛋白标准品可能适合其他色谱方法，如高效液相色谱(HPLC)。但是，某些缓冲体系可能会影响白蛋白和碳酸酐酶的洗脱体积。该试剂盒中的蛋白分子量范围为29 kDa至699 kDa。
其他說明
- 仅供研究使用。不可用于药物、家庭或其他用途。
- 有关危险和安全处理方法的信息，请参阅安全数据说明书。
- 有关试剂、保存和稳定性、实验方法的信息，请参阅产品信息说明书(product information sheet)。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- A8531Albumin, bovine serum, 66,000 MW 1 mL/vial化学品安全说明书
- A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vial化学品安全说明书
- A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vial化学品安全说明书
- A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vial化学品安全说明书
- D4772Blue dextran, 2,000,000 MW 1 mL/vial化学品安全说明书
- C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vial化学品安全说明书
- T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vial化学品安全说明书
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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