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Merck

T7572

Sigma-Aldrich

Thrombin 来源于人类血浆

400-1000 NIH units/mg protein

别名:

IIa因子

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

400-1000 NIH units/mg protein

雜質

HIV, hepatitis B and hepatitis C, tested negative

UniProt登錄號

儲存溫度

−20°C

基因資訊

human ... F2(2147)

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一般說明

冻干粉末,含有蔗糖、氯化钠和Tris。
凝血酶是止血过程中的最终凝血蛋白酶,可同时促进促凝血和抗凝血作用。

應用

凝血酶被用于重组融合蛋白的位点特异性切割,这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶已应用于一项研究,以评估用于小尺寸血小板微粒的流式细胞术的创新方法。

生化/生理作用

选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。

單位定義

活性以NIH单位表示,它是通过与NIH凝血酶参考标准品直接对比而获得的

分析報告

NIH测定程序根据Biggs改进方法,使用0.2ml稀释血浆(与生理盐水1:1)作为底物以及0.1ml凝血酶样本(在1%缓冲白蛋白溶液中稳定)。仅在15-25 秒的凝血时间内测定凝血酶浓度。

其他說明

请访问www.sigma-aldrich.com/enzymeexplorer查看有关凝血酶的更多信息。

免責聲明

仅供研究使用。本品在法国的科研应用受到监管,包括用于进口和出口活动(法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2)。如《公共卫生法典》所述,购买者(即最终用户)需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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At the cutting edge: conformational PARtiality.
T Madhusudhan et al.
Journal of thrombosis and haemostasis : JTH, 10(8), 1672-1674 (2012-06-30)
Silvia Montoro-García et al.
Thrombosis and haemostasis, 108(2), 373-383 (2012-06-29)
Microparticles (MPs) are small submicron membrane-derived vesicles shed from a variety of cells and they have been implicated in different disorders. Accordingly, understanding of physiological characteristics of MPs and improvement of methods of their quantification are important for further advance
Walter-Michael Halbmayer et al.
Clinical chemistry and laboratory medicine, 50(9), 1601-1605 (2012-10-27)
Dabigatran etexilate is a new oral anticoagulant for the therapy and prophylaxis of venous thromboembolism and stroke prevention in patients with atrial fibrillation. To investigate the extent of interactions of this new anticoagulant with frequently used coagulation assays, we completed
Herbert Nar
Trends in pharmacological sciences, 33(5), 279-288 (2012-04-17)
The quest for novel medications to treat thromboembolic disorders such as venous thrombosis, pulmonary embolism and stroke received a boost when the 3D structures of two major players in the blood coagulation cascade were determined in 1989 and 1993. Structure-guided
Lukas Graf et al.
Swiss medical weekly, 142, w13684-w13684 (2012-10-06)
New oral anticoagulants used as single target inhibitors of coagulation enzymes have been developed and tested in extensive trial programmes. Results of most of these trials showed non-inferior and/or superior efficacy and safety compared to standard treatment with LMWH or

商品

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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