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形狀
lyophilized powder
品質等級
比活性
≥2,000 NIH units/mg protein (E1%/280 = 19.5)
分子量
heavy chain ~33 kDa
light chain ~5 kDa
UniProt登錄號
應用
diagnostic assay manufacturing
儲存溫度
−20°C
基因資訊
cow ... F2(280685)
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生化／生理作用
丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。
單位定義
活性以NIH单位表示，通过与NIH凝血酶参考标准品直接对比确定。
外觀
从生理盐水柠檬酸钠缓冲液（pH 6.5）冻干
分析報告
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。
其他說明
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
基底
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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U Seligsohn
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商品
Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
实验方案
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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