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Merck

T1323

Sigma-Aldrich

Monoclonal Anti-ε-Tubulin antibody produced in mouse

clone TUB-11, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

TUB-11, monoclonal

形狀

buffered aqueous solution

分子量

antigen 55 kDa

物種活性

frog, mouse, chicken, human

技術

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured chicken fibroblasts

同型

IgG2a

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... TUBE1(51175)
mouse ... Tube1(71924)

一般說明

Monoclonal Anti-ε-Tubulin (mouse IgG2a isotype) is derived from the TUB-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide.
Tubulin epsilon 1 (ε-tubulin) is a member of tubulin superfamily. ε-tubulin is localized to the centrosome in cell-cycle-dependent manner. It is encoded by the gene mapped to human chromosome 6q21.

特異性

Monoclonal Anti-ε-Tubulin recognizes human, mouse, frog, and chicken ε-tubulin (55 kDa).

免疫原

synthetic peptide corresponding to amino acids 352-366 of human ε-tubulin, conjugated to KLH.

應用

Monoclonal Anti-ε-Tubulin antibody produced in mouse may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunofluorescence

生化/生理作用

Tubulin ε 1 (ε-Tubulin) plays a vital role in microtubule anchoring and centriole maturation.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ε-Tubulin Is an Essential Component of the Centriole
Dutcher SK
Molecular Biology of the Cell, 13, 3859?3869-3859?3869 (2002)
S Doxsey
Nature reviews. Molecular cell biology, 2(9), 688-698 (2001-09-05)
Over the past 100 years, the centrosome has risen in status from an enigmatic organelle, located at the focus of microtubules, to a key player in cell-cycle progression and cellular control. A growing body of evidence indicates that centrosomes might
Centrosome composition and microtubule anchoring mechanisms
Bornens M.
Current Opinion in Cell Biology, 14, 25-34 (2002)
Paul Chang et al.
Nature cell biology, 5(1), 71-76 (2003-01-03)
Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified epsilon-tubulin as a new member of the tubulin superfamily that localizes asymmetrically to
P G McKean et al.
Journal of cell science, 114(Pt 15), 2723-2733 (2001-10-31)
Although most eukaryotic cells can express multiple isotypes of alphabeta-tubulin, the significance of this diversity has not always been apparent. Recent data indicate that particular alphabeta-tubulin isotypes, both genome encoded and those derived by post-translational modification, can directly influence microtubule

商品

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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