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用途
sufficient for 10 purifications
品質等級
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技術
RNA purification: suitable
環保替代類別
, Aligned
儲存溫度
15-25°C
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相关类别
一般說明
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。本产品符合固有安全化学原则(Inherently Safer Chemistry)
Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式，可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。
在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。
从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。
从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
特點和優勢
- 每次制备至多可获得60 μg高纯度浓缩RNA
- 实验方案高度有效，可在30分钟内完成RNA纯化
- 专为研究困难的植物组织而设计
法律資訊
Spectrum is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
標靶器官
Liver,Heart
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
閃點（°F）
154.4 °F
閃點（°C）
68 °C
其他客户在看
Elsa Góngora-Castillo et al.
Methods in enzymology, 517, 139-159 (2012-10-23)
Development of next-generation sequencing, coupled with the advancement of computational methods, has allowed researchers to access the transcriptomes of recalcitrant genomes such as those of medicinal plant species. Through the sequencing of even a few cDNA libraries, a broad representation
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Frontiers in plant science, 11, 272-272 (2020-04-08)
Soybean [Glycine max (L.) Merr.] is an important crop for oil and protein resources worldwide, and its farming is impacted by increasing soil salinity levels. In Arabidopsis the gene EARLY FLOWERING 3 (ELF3), increased salt tolerance by suppressing salt stress
De novo Assembly of Leaf Transcriptome in the Medicinal Plant Andrographis paniculata.
Cherukupalli N
Frontiers in Plant Science, 7, 1203-1203 (2016)
Genomic approaches for interrogating the biochemistry of medicinal plant species.
Gongora-Castillo E
Methods in Enzymology, 517, 139-159 (2012)
Doug J Hinchliffe et al.
BMC genomics, 12, 445-445 (2011-09-10)
Cotton fiber length is an important quality attribute to the textile industry and longer fibers can be more efficiently spun into yarns to produce superior fabrics. There is typically a negative correlation between yield and fiber quality traits such as
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