SHC014

MISSION^® 对照载体

Contains a gene encoding TurboGFP, under the control of the UbC promoter

• 别名: MISSION^® Control Vectors
• MDL號碼: MFCD07785395
• 分類程式碼代碼: 41106609
• NACRES: NA.51

属性

• 品質等級: 200
• 產品線: MISSION^®
• 濃度: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

應用

To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Vector is a 9092 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the UbC promoter. The UbC-Turbo-GFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.

法律資訊

使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable