推荐产品
正在寻找类似产品? 访问 产品对比指南
一般說明
MISSION® TurboGFP™对照载体是一种 8347 碱基对的慢病毒质粒载体，其中包含编码 TurboGFP 的基因，由 CMV 启动子控制。TurboGFP 对照载体可用作 MISSION shRNA 文库克隆实验中的阳性转染/转导对照。TurboGFP™是从桡足类羽小角水蚤（Pontellina plumata） 克隆的绿色荧光蛋白 copGFP 的改良变异体。
氨苄青霉素和嘌呤霉素抗生素抗性基因分别用于细菌和哺乳动物细胞的选择性筛选。此外，利用表达载体与相容包装质粒共转染包装细胞(HEK293T)，可生产自灭活复制缺陷病毒颗粒。TurboGFP对照载体为溶于Tris-EDTA(TE)缓冲液的500 ng/μl溶液，含有10 μg质粒DNA。
氨苄青霉素和嘌呤霉素抗生素抗性基因分别用于细菌和哺乳动物细胞的选择性筛选。此外，利用表达载体与相容包装质粒共转染包装细胞(HEK293T)，可生产自灭活复制缺陷病毒颗粒。TurboGFP对照载体为溶于Tris-EDTA(TE)缓冲液的500 ng/μl溶液，含有10 μg质粒DNA。
應用
MISSION® pLKO.1-puro-CMV-TurboGFP ™ 阳性对照质粒 DNA 已被用于构建 GATA2 过表达载体 (oeG2)，方法是在 5′ 端去掉该载体的 GFP 盒。NheI 和 3′PstI 克隆位点，并用全长 GATA2 转录序列替代。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna
法律資訊
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
推薦
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Inactivation of SDH and FH cause loss of 5hmC and increased H3K9me3 in paraganglioma/pheochromocytoma and smooth muscle tumors.
Hoekstra AS
Oncotarget, 6(36), 38777-38788 (2015)
Selma Zargari et al.
BMC cancer, 20(1), 789-789 (2020-08-21)
MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have established pluripotency
Min Joo Kim et al.
Scientific reports, 9(1), 13288-13288 (2019-09-19)
Although various chemokines have pro-tumorigenic actions in cancers, the effects of CXCL16 remain controversial. The aim of this study was to investigate the molecular characteristics of CXCL16-expressing papillary thyroid cancers (PTCs). CXCL16 expressions were significantly higher in PTCs than benign
Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.
Diaz B
Oncotarget, 7(32), 51569-51580 (2016)
Reihaneh Alsadat Mahmoudian et al.
Molecular genetics & genomic medicine, 7(7), e00746-e00746 (2019-05-16)
MEIS1 (Myeloid ecotropic viral integration site 1), as a homeobox (HOX) transcription factor, has a dual function in different types of cancer. Although numerous roles are proposed for MEIS1 in differentiation, stem cell function, gastrointestinal development and tumorigenesis, the involved
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持