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重組細胞
expressed in E. coli
化驗
≥80% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
分子量
28 kDa
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... RASGEF1B(153020)
一般說明
Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.
應用
Human Ras has been used as a negative control in tubulin overlay assay.
生化／生理作用
Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.
包裝
Package size based on protein content
外觀
Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
Jean-Luc D
The Journal of Biological Chemistry (1997)
Slow conformational dynamics of the guanine nucleotide-binding protein Ras complexed with the GTP analogue GTPgammaS.
Spoerner M
FEBS Journal (2007)
J Tucker et al.
The EMBO journal, 5(6), 1351-1358 (1986-06-01)
v-Ha-ras encoded p21 protein (p21V), the cellular c-Ha-ras encoded protein (p21C) and its T24 mutant form p21T were produced in Escherichia coli under the control of the tac promoter. Large amounts of the authentic proteins in a soluble form can
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Shutes A, et al.
Test (2006)
Structure and function of p21 ras proteins
Shih TY, et al.
Gene Analysis Techniques (1986)
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