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Merck

PIC0004

Sigma-Aldrich

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution

别名:

Protease inhibitor cocktail

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.28

product name

ReadyShield®蛋白酶抑制剂Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution

描述

Purification of Histidine-Tagged Proteins

形狀

ready-to-use solution

品質

200

包裝

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

儲存溫度

−20°C

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一般說明

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

特點和優勢

  • Does not freeze in storage for quick, convenient use.
  • Tailored for use with Histidine fusion tagged proteins.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

準備報告

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

其他說明

For life science research only. Not for use in diagnostic procedures.

法律資訊

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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