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Merck

P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.77

生物源

synthetic

品質等級

形狀

DMSO solution

儲存溫度

−20°C

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一般說明

蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。

溶液中混有多种抑制剂成分,可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。

特異性

抑制丝氨酸,半胱氨酸,天冬氨酸,和嗜热菌蛋白酶样蛋白酶和氨基肽酶。

應用

混合液经过专门的优化和验证,适合分离组氨酸标签(His-tag)蛋白质,特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。

推荐用量:1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。

特點和優勢

专门为组氨酸标签蛋白纯化而配制。

靶向多种蛋白酶,具有全面的蛋白酶抑制活性。

不加螯合剂,适合IMAC应用。

方便的包装选择。

成分

AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮

數量

推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。

该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试

準備報告

该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

185.0 °F - closed cup

閃點(°C)

85 °C - closed cup


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C Rivier et al.
The EMBO journal, 20(7), 1765-1773 (2001-04-04)
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The physical interaction between the presynaptic vesicle release complex and the large cytoplasmic region linking domains II and III of N-type (Ca(v)2.2) calcium channel alpha(1)B subunits is considered to be of fundamental importance for efficient neurotransmission. By PCR analysis of
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ASPP2 is a key protein in regulating apoptosis both in p53-dependent and-independent pathways. The C-terminal part of ASPP2 contains four ankyrin repeats and an SH3 domain (Ank-SH3) that mediate the interactions of ASPP2 with apoptosis related proteins such as p53
E Boudreau et al.
The EMBO journal, 19(13), 3366-3376 (2000-07-06)
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of

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