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Merck

MAK138

Sigma-Aldrich

自噬检测试剂盒

sufficient for 200 fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 200 fluorometric tests

檢測方法

fluorometric

相關疾病

cancer; immunological diseases

儲存溫度

−20°C

一般說明

自噬是一种进化上保守的降解过程,其降解目标为长寿命蛋白质、细胞器和其他细胞质成分,其通过溶酶体途径进行降解。多重细胞作用需要激活自噬途径,包括饥饿期间的存活、细胞内组分的清除、发育、以及免疫。

應用


  • Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.: This study explores the inhibitory effects of ursolic acid on the proliferation and migration of renal carcinoma cells. The use of an autophagy assay kit was instrumental in demonstrating that ursolic acid induces autophagy, leading to decreased cell viability and migration. These findings highlight the potential of ursolic acid as a therapeutic agent in renal carcinoma through the modulation of autophagy (Lyu et al., 2022).

適合性

本试剂盒适用于荧光显微镜和荧光微板分析仪检测哺乳动物细胞的自噬情况。

原則

自噬检测试剂盒提供了一种简单直接的方法,使用专有的荧光自噬体标记物(λex = 333 /λem = 518nm)对多种细胞类型的自噬进行测定。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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Autophagy in the pathogenesis of disease.
Levine B and Guido K
Cell, 132(1), 27-42 (2008)
Autophagy: machinery and regulation.
Yin Z, et al.
Microbial cell, 3(12), 588-588 (2016)
Ilenia Martinelli et al.
Redox biology, 40, 101866-101866 (2021-01-26)
Autophagy and apoptosis are powerful regulators of multiple facets of cellular metabolism and homeostasis. Here, we uncover that galanin, a pleiotropic peptide, regulates cardiac autophagy and deactivates apoptotic cell death through the Forkhead box protein O1 (FoxO1) pathway. In hypertrophied
Simin Nazarnezhad et al.
Neurotoxicology, 74, 196-202 (2019-07-20)
Diabetic neuropathy (DN) is the most common complication of diabetes mellitus. It is thought that neuronal cell death which is mainly due to reactive oxygen species (ROS) overproduction in the cells is responsible for most symptoms of this disorder. Nesfatin-1
Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage.
Moussa M, et al.
Experimental Cell Research, 352(1), 146-156 (2017)

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