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Merck

M7383

Sigma-Aldrich

苹果酸脱氢酶 来源于猪心脏

≥400 units/mg protein (biuret), ammonium sulfate suspension

别名:

L-苹果酸: NAD+ 氧化还原酶, MDH, Malate Dehydrogenase

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

ammonium sulfate suspension

比活性

≥400 units/mg protein (biuret)

異物活動

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

儲存溫度

2-8°C

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一般說明

Cytoplasmic Isozyme

應用

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.

生化/生理作用

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.

單位定義

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

外觀

Suspension in 3.2 M (NH4)2SO4, 0.1 M KH2PO4, pH 7.0

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析证书(COA)

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M L Sánchez-Martínez et al.
Talanta, 78(1), 305-309 (2009-01-29)
A simple and rapid homogeneous enzyme immunoassay involving the use of the malic dehydrogenase enzyme and a long-wavelength fluorophor, the oxazine Cresyl Violet, is proposed for the determination of the antibiotic amikacin in water samples. An enzymatic tracer has been
P Benoist et al.
Analytical biochemistry, 187(2), 337-344 (1990-06-01)
Nanogram amounts of soluble aminopeptidases, dehydrogenases, and esterases were detected by nondenaturing ultralow gelling point agarose-polyacrylamide gel electrophoresis (ULGA-PAGE). Cytosolic fractions from Frankia sp. were electrophoresed at 4 degrees C in the presence of Co2+, Zn2+, or Mg2+ ions. Then
Ethel V Larregle et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 46(5), 1786-1792 (2008-03-11)
We investigated the effect of exposition to cadmium (Cd, 15ppm for 8 weeks) through drinking water on liver lipid metabolism in adult male Wistar rats. As compared to metal non-exposed (control) rats, the serum triglycerides, cholesterol and LDL+VLDL cholesterol concentrations
Marie Knockaert et al.
The Journal of biological chemistry, 277(28), 25493-25501 (2002-04-20)
Numerous inhibitors of cyclin-dependent kinases and glycogen synthase kinase-3 (GSK-3) are being developed in view of their potential applications against cancers and neurodegenerative disorders. Among these, paullones constitute a family of potent and apparently selective cyclin-dependent kinase and GSK-3 inhibitors.
K Smith et al.
Biochimica et biophysica acta, 884(1), 109-118 (1986-10-29)
The cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (CTAB), completely inactivates porcine heart cytoplasmic malate dehydrogenase (L-malate:NAD+ oxidoreductase, EC 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. These concentrations are close to, or higher

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