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生物源
Porcine heart
形狀
ammonium sulfate suspension
比活性
≥100 units/mg protein
分子量
98 kDa ( 49 kDa monomer)
溶解度
H2O: soluble 1.0 mg/mL, clear
異物活動
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
儲存溫度
2-8°C
應用
猪心脏来源柠檬酸合酶用于：
- 注入蝾螈卵子，确定受精时，其在卵子激活中的重要性
- 制备反应混合物，确定丙酮酸羧化酶活性
- 检查其是否能够诱导未受精卵中的[Ca22+]增加和卵子激活
生化／生理作用
柠檬酸合成酶催化柠檬酸在辅酶 A 存在下转化为乙酰辅酶 A，释放 H2O和草酰乙酸。该酶分子量为 85 kDa，pI 为 6.1-6.6。可被氟乙酰辅酶 a、棕榈酰辅酶 a 和柠檬酸辅酶 a 抑制。当其被乙酸酐乙酰化或被碘碘化时也受到抑制。
單位定義
一个装置将形成 1.0μ37°C 下 pH 8.0 时每分钟由草酰乙酸和乙酰 CoA 生成的柠檬酸盐摩尔数。
外觀
用 3.2 M(NH4)2SO4 溶液 (pH 7.0) 混悬。
準備報告
溶于水，形成 1 mg/mL 浓度的澄清溶液。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Florian Stengel et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(5), 2007-2012 (2010-02-06)
Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular stress. Here we probe the architecture and dynamics of complexes formed between an oligomeric sHSP and client by
Oded Rimon et al.
Antioxidants & redox signaling, 27(15), 1252-1267 (2017-04-11)
A recently discovered group of conditionally disordered chaperones share a very unique feature; they need to lose structure to become active as chaperones. This activation mechanism makes these chaperones particularly suited to respond to protein-unfolding stress conditions, such as oxidative
Xiangjian Gou et al.
Plant biotechnology journal, 20(7), 1417-1431 (2022-04-11)
Single amino acid substitution (SAAS) produces the most common variant of protein function change under physiological conditions. As the number of SAAS events in plants has increased exponentially, an effective prediction tool is required to help identify and distinguish functional
Skylar Xantus Kim et al.
eLife, 7 (2018-07-17)
Anhydrobiotes are rare microbes, plants and animals that tolerate severe water loss. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, budding yeast, we
Alterations in Cytosolic and Mitochondrial [U-13C] Glucose Metabolism in a Chronic Epilepsy Mouse Model
McDonald TS, et al.
eNeuro, 4(1), 266-276 (2017)
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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