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品質等級
無菌
0.2 μm filtered
形狀
lyophilized powder
比活性
≥4000 units/mg protein (biuret)
分子量
23 kDa
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成,可能是一种有效的龋病防治剂。
應用
为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。
生化/生理作用
变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶,可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。
單位定義
以粪链球菌细胞壁混悬液为底物,在1mL体积中,1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600 。
外觀
含聚蔗糖和琥珀酸钠缓冲盐的冻干粉
準備報告
由 M 9901 制备
其他說明
查看更多关于 复合糖分析用酶的信息, 请访问 www.sigma-aldrich.com/enzymeexplorer
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
This procedure may be used for Mutanolysin products.
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