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Merck

M4782

Sigma-Aldrich

变溶菌素 来源于球孢链霉菌 ATCC 21553

0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

品質等級

無菌

0.2 μm filtered

形狀

lyophilized powder

比活性

≥4000 units/mg protein (biuret)

分子量

23 kDa

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

链霉菌 中提取的变溶菌素由两种主要的裂解酶组成,可能是一种有效的龋病防治剂。

應用

为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。 它已可用于形成球状体以分离DNA。

生化/生理作用

变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶,可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌,如乳杆菌属和乳球菌属。

單位定義

粪链球菌细胞壁混悬液为底物,在1mL体积中,1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA600

外觀

含聚蔗糖和琥珀酸钠缓冲盐的冻干粉

準備報告

由 M 9901 制备

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Journal of bacteriology, 188(3), 902-908 (2006-01-24)
The O acetylation of peptidoglycan occurs specifically at the C-6 hydroxyl group of muramoyl residues. Using a combination of high-performance liquid chromatography-based organic acid analysis and carbohydrate analysis by high-pH anion-exchange chromatography, we determined that strains of Entercoccus durans, E.
Carli B Jones et al.
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Short read 16 S rRNA amplicon sequencing is a common technique used in microbiome research. However, inaccuracies in estimated bacterial community composition can occur due to amplification bias of the targeted hypervariable region. A potential solution is to sequence and assess
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Fliss, I., et al.
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According to the current insights, the predominant bacterial community in human feces is considered to be stable and unique for each individual over a prolonged period of time. In this study, the temporal stability of both the predominant population and
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal

实验方案

This procedure may be used for Mutanolysin products.

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