生物源
bacterial (Arthrobacter luteus)
品質等級
形狀
lyophilized powder
比活性
≥2,000 units/mg protein
成份
Protein, ≥20% biuret
技術
cell based assay: suitable
適合性
suitable for cell lysis
應用
diagnostic assay manufacturing
儲存溫度
−20°C
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應用
藤黄节杆菌（Arthrobacter luteus ）裂解酶已用于裂解真菌细胞壁，分离DNA。
Lyticase from Arthrobacter luteus has been used:
- for spheroplasting the cells
- as a component of digestion solution to incubate yeast cells for digestion of the cell wall
- in the enzymatic hydrolysis of the mycelium precipitate to prepare protoplasts
生化／生理作用
酵母细胞很难被破坏，因为其细胞壁可能形成胶囊或耐药孢子。可通过裂解酶（如溶壁酶）诱导部分原生质球形成，从酵母中提取DNA。随后再裂解原生质球以释放DNA。溶壁酶更易消化酵母菌细胞壁，并从真菌中产生原生质球进行转化。它含有β-(1→3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。溶壁酶适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units. Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria. The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
單位定義
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
外觀
部分纯化的冻干粉末，含有磷酸钾缓冲盐和稳定剂
其他說明
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
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The interplay between chromatin structure and DNA topology is a fundamental, yet elusive, regulator of genome activities. A paradigmatic case is the "linking number paradox" of nucleosomal DNA, which refers to the incongruence between the near two left-handed superhelical turns
实验方案
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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