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Merck
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主要文件

M1570

Sigma-Aldrich

Anti-MYH-1 Antibody

enhanced validation

mouse monoclonal, MY-32

别名:

单克隆抗肌球蛋白（骨骼肌快肌） 小鼠抗

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About This Item

MDL號碼:
MFCD00145920
分類程式碼代碼:
12352203
NACRES:
NA.41

产品名称

抗肌球蛋白(骨骼肌,快速)抗体，小鼠单克隆抗体, clone MY-32, purified from hybridoma cell culture

生物源

mouse

品質等級

200

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

MY-32, monoclonal

形狀

buffered aqueous solution

物種活性

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

包裝

antibody small pack of 25 μL

加強驗證

independent
Learn more about Antibody Enhanced Validation

濃度

~1.0 mg/mL

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue
microarray: suitable
western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle

同型

IgG1

UniProt登錄號

P12882

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... MYH1(4619) , MYH2(4620)
mouse ... Myh1(17879) , Myh2(17882)
rat ... Myh1(287408) , Myh2(691644)

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相关类别

一般說明

定位于肌球蛋白重链上的表位。可对骨骼肌中发现的快速（II型）和新生儿肌动蛋白分子进行染色，但不会对培养细胞中的心肌、平滑肌或非肌肉肌球蛋白染色。能与人类横纹肌肉瘤发生反应。

免疫原

兔肌肉肌球蛋白。

應用

使用单克隆小鼠抗肌球蛋白（骨骼/快速）作为一抗，以1：90000的稀释度，通过蛋白质印迹对来自具有过去损伤的运动员的血清样品中的肌球蛋白（快）水平进行测定。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫组织化学（1篇论文）

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
22190705
119M4775V
079M4861V
018M4882V
22180118

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Charlotte Capitanchik et al.
Nucleus (Austin, Tex.), 9(1), 410-430 (2018-06-19)
Laminopathies yield tissue-specific pathologies, yet arise from mutation of ubiquitously-expressed genes. A little investigated hypothesis to explain this is that the mutated proteins or their partners have tissue-specific splice variants. To test this, we analyzed RNA-Seq datasets, finding novel isoforms
Yanlin Wang et al.
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, 40(6), 1255-1265 (2019-03-21)
Myotonic dystrophy type 1 (DM1) is caused by CTG nucleotide repeat expansions in the 3'-untranslated region (3'-UTR) of the dystrophia myotonica protein kinase (DMPK) gene. The expanded CTG repeats encode toxic CUG RNAs that cause disease, largely through RNA gain-of-function.
Tomohiko Akiyama et al.
Scientific reports, 8(1), 1189-1189 (2018-01-21)
Direct generation of skeletal muscle cells from human pluripotent stem cells (hPSCs) would be beneficial for drug testing, drug discovery, and disease modelling in vitro. Here we show a rapid and robust method to induce myogenic differentiation of hPSCs by
Maegen A Ackermann et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 27(8), 3217-3228 (2013-05-10)
Myosin binding protein C (MyBP-C) is expressed in striated muscles, where it plays key roles in the modulation of actomyosin cross-bridges. Slow MyBP-C (sMyBP-C) consists of multiple variants sharing common domains but also containing unique segments within the NH2 and
Jin Young Lee et al.
Frontiers in cell and developmental biology, 8, 565826-565826 (2020-11-27)
Skeletal muscle and bone are highly interrelated, and previous proteomic analyses suggest that lumican is one of muscle-derived factors. To further understand the role of lumican as a myokine affecting adjacent bone metabolism, we investigated the effects of lumican on
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