L6016

Lipopolysaccharides (rough strains) from Salmonella enterica serotype typhimurium TV119 (Ra mutant)

• 别名: LPS
• MDL號碼: MFCD00164404
• 分類程式碼代碼: 12352201
• NACRES: NA.25

属性

• 生物源: Salmonella enterica (Serotype typhimurium TV119 Ra mutant)
• 形狀: lyophilized powder
• 雜質: <3% Protein (Lowry)
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

说明

一般說明

This product is phenol:chloroform:petroleum ether extracted from Salmonella typhimurium strain TV119(Ra mutant). The source strain is from a private collection.

生化／生理作用

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

準備報告

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

其他說明

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

安全信息

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)