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Key Documents

L0543

Sigma-Aldrich

LB Agar Kanamycin-50, Plates

pre-poured LB agar plates with 50 ug/ml kanamycin

别名:

kanamycin agar plates

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About This Item

分類程式碼代碼:
41121800

等級

for molecular biology

無菌

sterile; autoclaved

形狀

plates (10 cm)
plates (10cm, 20-25ml each)

容量

20-25 mL

儲存溫度

2-8°C

適合性

selective for Escherichia coli
selective for coliforms

一般說明

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

應用

Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.

特點和優勢

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

成分

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
Deich RA, et al.
Journal of Bacteriology, 170(2), 489-498 (1988)
Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles
Talhinhas P, et al.
Molecular Biotechnology, 39(1), 57-67 (2008)

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