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一般說明
BL21（DE3）化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因，可用于含有T7启动子的任何表达质粒。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
應用
该 大肠杆菌 BL21（DE3）菌株已用于：以pET19b作为表达载体，表达tau 2N4R同种型（aa1–441）和截短的tau（aa1–421）。BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。
特點和優勢
- 转化效率> 1×107 CFU /µg
- 实惠的价格
- 可为日常蛋白表达工作提供卓越价值。
成分
- BL21（DE3）化学感受态细胞
- pUC19转化对照DNA
- 表达恢复培养基
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价格
儲存類別代碼
10 - Combustible liquids
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