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Key Documents

CMC0017

Sigma-Aldrich

过表达 ™ C41 (DE3) 化学感受态细胞

Escherichia coli, rod shaped

别名:

C41 strain

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.85

product name

过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression

生物源

Escherichia coli

等級

for molecular biology

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

相关类别

一般說明

OverExpress™ 电感受态和化学感受态细胞是可以有效表达所有种类生物体（包括真细菌、酵母、植物、病毒和哺乳动物）的有毒蛋白质的大肠杆菌。这些新菌株表达有毒蛋白质的有效性已在350多篇论文中得到验证。OverExpress™菌株含有从表型上选择的基因突变，以产生对有毒蛋白质的耐受性。C41(DE3) 菌株来源于 BL21(DE3)。该菌株至少有一处突变，其防止了与许多重组有毒蛋白表达相关的细胞死亡。菌株C43（DE3）来源于C41（DE3），是通过选择对不同毒性蛋白的抗性而得到的，并且可以表达一组不同的C41（DE3）的毒性蛋白。OverExpress C41（DE3）pLysS和C43（DE3）pLysS还带有抗氯霉素的质粒，该质粒可编码T7溶菌酶，其是T7 RNA聚合酶的一种天然抑制剂。含有pLysS的细胞会产生少量的T7溶菌酶。这些菌株用于抑制诱导以前T7 RNA聚合酶的基础表达，从而使编码特别有毒的蛋白质的重组体保持稳定。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

應用

过表达 ™ C41 (DE3) 化学感受态细胞已用于表达筛选。

特點和優勢

  • 用这些 BL21 (DE3) 衍生物克隆到任何 T7 载体的表达基因
  • 有效表示毒性和膜蛋白类
  • 被超过 350 篇研究文章引用

成分

  • 过表达 C41 (DE3) 化学感受态细胞
  • pUC 19 转化对照 DNA
  • 表达回收培养基


法律資訊

OverExpress is a trademark of Imaxio, S.A.

儲存類別代碼

10 - Combustible liquids

分析证书(COA)

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Rosetta 2(DE3) 感受态细胞 - Novagen Novagen′s Rosetta 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71397

Rosetta 2(DE3) 感受态细胞 - Novagen

A high-throughput expression screening platform to optimize the production of antimicrobial peptides.
Schreiber C, et al.
Microbial cell factories, 16(1), 29-29 (2017)
Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair.
Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in
Myles B C Dillon et al.
The Journal of biological chemistry, 288(39), 27872-27880 (2013-08-16)
Protein arginine methyltransferase (PRMT) 8 is unique among the PRMTs, as it has a highly restricted tissue expression pattern and an N terminus that contains two automethylation sites and a myristoylation site. PRMTs catalyze the transfer of a methyl group
Neale Harrison et al.
PloS one, 9(11), e113007-e113007 (2014-11-26)
N-acylethanolamines are an important class of lipid signaling molecules found in many species, including the nematode Caenorhabditis elegans (C. elegans) where they are involved in development and adult lifespan. In mammals, the relative activity of the biosynthetic enzyme N-acyl phosphatidylethanolamine-specific

实验方案

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

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