I5271

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 别名: IL-1β, Lymphocyte activating factor
• MDL號碼: MFCD00164815
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• product name: Interleukin-1β from mouse, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 化驗: ≥97% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: ≤0.010 ng/mL ED₅₀
• 品質: endotoxin tested
• 分子量: 17.5 kDa (containing 153 amino acid residues)
• 包裝: pkg of 5X5 μg; pkg of 5 μg
• 技術: cell culture | mammalian: suitable
• 雜質: ≤0.1ng/mg
• UniProt登錄號: P10749
• 儲存溫度: −20°C
• 基因資訊: mouse ... Il1b(16176)

说明

一般說明

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.

應用

Interleukin-1β from mouse has been used:

• as a proinflammatory agent, to induce inflammatory signaling cascades mediated cellular damage
• to study its effect on fetuin A-induced inflammatory response in podocyte
• to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes

生化／生理作用

Interleukin-1β (IL-1β) is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

外觀

Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0

分析報告

The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells.

其他說明

The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable