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種類
Type III
形狀
lyophilized powder
比活性
≥25 units/mg protein (biuret)
分子量
dimer 110 kDa
異物活動
glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
儲存溫度
−20°C
基因資訊
bakers yeast ... HXK1(850614) , HXK2(852639)
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生化/生理作用
以ATP作为磷酸盐来源,催化C6位的 D-己糖的磷酸化。
不同的己糖具有不同的磷酸化速率(pH 7.5,30 °C)。
D-果糖 KM:0.33 mM
D-葡萄糖 KM:0.12 mM
D-甘露糖 KM:0.05 mM
酵母己糖激酶具有两种相似的亚型,即PI和PII(A和B), 其等电点分别为5.25和4。
分子量: ~ 54 kDa (单体)
~110 kDa (二聚体)
最适pH:7.5至9.0
消光系数:在280 nm处,E1% = 8.85 (PI)和9.47 (PII)
活化剂:己糖激酶的活化需要 Mg2+ 离子(KM = 2.6 mM)。 己糖激酶可被儿茶酚胺及其相关化合物激活。
抑制剂:山梨糖-1-磷酸,多磷酸盐,6-脱氧-6-氟葡萄糖,2-C-羟基-甲基葡萄糖,木糖,来苏糖和硫醇反应性化合物(Hg2+ 和4-氯代苯甲酸酯)
不同的己糖具有不同的磷酸化速率(pH 7.5,30 °C)。
D-果糖 KM:0.33 mM
D-葡萄糖 KM:0.12 mM
D-甘露糖 KM:0.05 mM
酵母己糖激酶具有两种相似的亚型,即PI和PII(A和B), 其等电点分别为5.25和4。
分子量: ~ 54 kDa (单体)
~110 kDa (二聚体)
最适pH:7.5至9.0
消光系数:在280 nm处,E1% = 8.85 (PI)和9.47 (PII)
活化剂:己糖激酶的活化需要 Mg2+ 离子(KM = 2.6 mM)。 己糖激酶可被儿茶酚胺及其相关化合物激活。
抑制剂:山梨糖-1-磷酸,多磷酸盐,6-脱氧-6-氟葡萄糖,2-C-羟基-甲基葡萄糖,木糖,来苏糖和硫醇反应性化合物(Hg2+ 和4-氯代苯甲酸酯)
單位定義
One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.
外觀
Lyophilized powder containing approx. 15% sodium citrate
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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