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Merck

H5000

Sigma-Aldrich

己糖激酶 来源于酿酒酵母

Type III, lyophilized powder, ≥25 units/mg protein (biuret)

别名:

ATP:D-己糖-6-磷酸转移酶, 己糖激酶 来源于酵母

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410

種類

Type III

形狀

lyophilized powder

比活性

≥25 units/mg protein (biuret)

分子量

dimer 110 kDa

UniProt登錄號

異物活動

glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%

儲存溫度

−20°C

基因資訊

bakers yeast ... HXK1(850614) , HXK2(852639)

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生化/生理作用

以ATP作为磷酸盐来源,催化C6位的 D-己糖的磷酸化。

不同的己糖具有不同的磷酸化速率(pH 7.5,30 °C)。
D-果糖 KM:0.33 mM
D-葡萄糖 KM:0.12 mM
D-甘露糖 KM:0.05 mM

酵母己糖激酶具有两种相似的亚型,即PI和PII(A和B), 其等电点分别为5.25和4。

分子量: ~ 54 kDa (单体)
~110 kDa (二聚体)
最适pH:7.5至9.0
消光系数:在280 nm处,E1% = 8.85 (PI)和9.47 (PII)

活化剂:己糖激酶的活化需要 Mg2+ 离子(KM = 2.6 mM)。 己糖激酶可被儿茶酚胺及其相关化合物激活。

抑制剂:山梨糖-1-磷酸,多磷酸盐,6-脱氧-6-氟葡萄糖,2-C-羟基-甲基葡萄糖,木糖,来苏糖和硫醇反应性化合物(Hg2+ 和4-氯代苯甲酸酯)

單位定義

One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.

外觀

Lyophilized powder containing approx. 15% sodium citrate

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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