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形狀
solid
特點
wetted part: no
包裝
pack of 25 g
製造商/商標名
Cytiva RPN6302
顏色
Yellowish
溶解度
cold water: soluble (Easily soluble in cold and hot water)
儲存溫度
2-8°C
一般說明
Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.
應用
Iodoacetamide has been used:
- as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
- in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
- as a protein alkylation agent
生化/生理作用
Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
特點和優勢
- Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
訊號詞
Danger
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
其他客户在看
Differential effects of iodoacetamide and iodoacetate on glycolysis and glutathione metabolism of cultured astrocytes
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Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction
Journal of drug and alcohol research, 6(3), b1-b8 (2014)
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实验方案
This page describes typical rehydration solutions for use in gel electrophoresis with GE Healthcare products.
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