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TALON® Superflow Cobalt-IMAC
包裝
pack of 50 mL
製造商／商標名
Cytiva 28-9575-02
基質
6% cross-linked agarose
粒徑
60-160 μm
cleaning in place
2-14(Co2+-stripped medium)
工作範圍
3-12(Co2+-stripped medium)
容量
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
儲存溫度
2-8°C
一般說明
特點和優勢
- Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page describes manual purification of histidine-tagged proteins using His GraviTrap TALON from GE Healthcare.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
实验方案
This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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