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Key Documents

GE29-0510-21

HisTrap High Performance

Cytiva 29-0510-21, pack of 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

包裝

pack of 1 mL

製造商/商標名

Cytiva 29-0510-21

存貨情形

not available in North America

參數

flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-14(Ni2+-stripped medium.)

工作範圍

3-12(Ni2+-stripped medium)

容量

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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一般說明

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

應用

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

特點和優勢

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

法律資訊

HisTrap is a trademark of Cytiva

象形圖

Environment

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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实验方案

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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