推荐产品
材料
polypropylene (PP)
分子量
1000-5000 Da (globular proteins)
包裝
pack of 4
製造商/商標名
Cytiva 17-5087-02
儲存條件
dry media
參數
<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)
床體積
53 mL
柱長度 × 內徑
10 cm × 26 mm
基質
cross-linked dextran
polymeric
基質活性組
dextran-epichlorohydrin phase
粒徑
17-132 μm (dry)
cleaning
2-13
工作範圍
2-13
分離技術
size exclusion (SEC)
一般說明
特點和優勢
- Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
- Replaces dialysis for desalting and therefore saves time and reduces labor.
- Compatible with a laboratory pump or chromatography system such as AKTA design.
- Two or four columns can be used in series for sample sizes of 30 to 60 mL.
適合性
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
Antibodies are needed for research and industrial purposes in different quantities, from microgram to kilogram scale. It is important to design and use a purification method that will yield protein of a quality and quantity that is adequate for the particular application.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
实验方案
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.
This page shows how to remove histidine tags by enzymatic cleavage using GE Healthcare products.
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
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